Crystallization of a Mos1 transposase-inverted-repeat DNA complex: biochemical and preliminary crystallographic analyses

A complex formed between Mos1 transposase and its inverted-repeat DNA has been crystallized. The crystals diffract to 3.25 Å resolution and exhibit monoclinic (P2(1)) symmetry, with unit-cell parameters a = 120.8, b = 85.1, c = 131.6 Å, β = 99.3°. The X-ray diffraction data display noncrystallographic twofold symmetry and characteristic dsDNA diffraction at ∼3.3 Å. Biochemical analyses confirmed the presence of DNA and full-length protein in the crystals. The relationship between the axis of noncrystallographic symmetry, the unit-cell axes and the DNA diffraction pattern are discussed. The data are consistent with the previously proposed model of the paired-ends complex containing a dimer of the transposase

Battling from the Bottom Up: The Alleviation of Violence Against Women by the Non-Governmental Sector in South Africa

This thesis seeks to examine the work of the Non-Governmental sector in alleviating violence against women in South Africa. The first chapter analyzes VAW in two time periods—1948-1994 and the post-Apartheid era, 1994-2002. The second chapter provides an overview of the roles and methodology of NGOs in post-Apartheid South Africa and presents a list of strategies that an effective NGO must employ. Finally, Chapter 3 is a case study of POWA (People Opposing Women Abuse), a Johannesburg-based VAW NGO. The case study uses the strategies and typology laid out in Chapter 2 to evaluate the work of POWA. The researcher utilized a combination of books, journal articles, websites, government documents, and quantitative research studies. For the case study, the researcher developed two questionnaires which were sent to two departments at POWA—the Training and Public Awareness department and the Information and Resource Center; the responses were compiled into two tables and several pages of text (Chapter 3). The study found that the NGO sector in South Africa is performing the ground work of fighting VAW—^providing counseling, legal aid, education workshops to abused women and advocating on their behalf as well as providing counseling and workshops to men, children, and even perpetrators. POWA is leading the coalition of NGOs that fight VAW in South Africa. Though POWA is often limited in both human and financial capital, it possesses the ability to change the current atmosphere of violence against women in South Afnca

Fetal heterotaxy with tricuspid atresia, pulmonary atresia, and isomerism of the right atrial appendages at 22 weeks.

We report the accurate prenatal diagnosis at 22 weeks gestation of right atrial isomerism in association with tricuspid atresia. Several distinctive sonographic features of isomerism of the right atrial appendages were present in this fetus: complex cardiac abnormality, ventriculoarterial discordance, juxtaposition of the aorta and the inferior vena cava to the right side, pulmonary atresia, and anomalous pulmonary venous return to the morphological right atrium. Tricuspid atresia, which is an extremely rare lesion within heterotaxy spectrum disorders, was present. Postnatal investigations confirmed all prenatally diagnosed abnormalities, with additional findings of pulmonary atresia with discontinuous pulmonary arteries and bilateral arterial ducts, asplenia, and bilateral eparterial bronchi. To our knowledge, tricuspid atresia in the setting of isomerism of the right atrial appendages has not previously been diagnosed or reported prenatally. Because of the complexity of cardiac lesions that may be present in cases of atrial isomerism, these disorders should be considered even if sonographic findings are uncommon or atypical

The ‘double-edged sword’ of a sessional academic career

There have been widespread changes to working arrangements and employment relationships, including significant decreases in continuing/full-time employment contracts. This trend is particularly notable in academia, with more universities relying on the expertise of sessional, teaching-focused academics. This qualitative study extends understanding of this important group of professionals, identifying sessional work as a ‘double-edged sword’ and suggesting a typology of sessional academic careers to be tested in future research. It reports on the diversity among sessional academics, some enjoying the autonomy and flexibility of this working arrangement, others seeking more job security and greater alignment with continuing employment. It also identifies synergies and contradictions between sessional academic careers and key themes in the contemporary careers literature

The Covid-19 crisis as a career shock: Implications for careers and vocational behavior

The covid-19 pandemic is a career shock for many people across the globe. In this article, we reflect on how insights from the literature on career shocks can help us understand the career consequences of the pandemic and offer suggestions for future research in this area. In particular, we offer three “key lessons”. The first lesson is that the implications of Covid-19 reflect the dynamic interplay between individual and contextual factors. Here, we argue that although the pandemic was difficult to predict and control, research shows that certain psychological resources – such as career competencies and resilience – could make this career shock more manageable. The second lesson is that the pandemic may have differential implications over time, as suggested by research that has shown the consequences of career shocks to differ between short-term vs. long-term time horizons, and across life- and career stages. The third lesson is that, even though the pandemic is clearly a negatively valenced shock for most people, further into the future it may allow for more positive outcomes. This lesson builds on research showing how negative career shocks have long-term positive consequences for some people. We hope that these insights will inspire both scholars and practitioners to study and understand the work and career implications of Covid-19 as a career shock, as well as to support people in dealing with its consequences

Digital intrusions or distraction at work and work-Life conflict

Internet usage for non-work activities during work hours is an increasingly common concern among management scholars and practitioners as well as for employees, particularly in relation to its impact on work-life conflict and individual well-being. Drawing on memory for goals theory, this study investigates the distinction between digital intrusions and digital interruptions during work and their concomitant impact on work-life conflict. Using a set-theoretic approach to analyse data gathered from information technology (IT) professionals in Germany and Australia, we explain how a 2 * 2 matrix comprising non-work online messaging, or personal digital communication, during work and intrusion contributes to work-life conflict. A key finding is that employees reported work-life conflict only if they perceived private messaging as a source of intrusion rather than as a distraction. From a practical perspective, this finding suggests that employers may provide employees with micro-breaks to attend to perceived intrusions and thus reduce sources of work-life conflict. Contributions and suggestions for future studies are discussed

A cohort study of the recovery of health and wellbeing following colorectal cancer (CREW study): protocol paper

Background: the number of people surviving colorectal cancer has doubled in recent years. While much of the literature suggests that most people return to near pre-diagnosis status following surgery for colorectal cancer, this literature has largely focused on physical side effects. Longitudinal studies in colorectal cancer have either been small scale or taken a narrow focus on recovery after surgery. There is a need for a comprehensive, long-term study exploring all aspects of health and wellbeing in colorectal cancer patients. The aim of this study is to establish the natural history of health and wellbeing in people who have been treated for colorectal cancer. People have different dispositions, supports and resources, likely resulting in individual differences in restoration of health and wellbeing. The protocol described in this paper is of a study which will identify who is most at risk of problems, assess how quickly people return to a state of subjective health and wellbeing, and will measure factors which influence the course of recovery. Methods: this is a prospective, longitudinal cohort study following 1000 people with colorectal cancer over a period of two years, recruiting from 30 NHS cancer treatment centres across the UK. Questionnaires will be administered prior to surgery, and 3, 9, 15 and 24 months after surgery, with the potential to return to this cohort to explore on-going issues related to recovery after cancer. Discussion: outcomes will help inform health care providers about what helps or hinders rapid and effective recovery from cancer, and identify areas for intervention development to aid this process. Once established the cohort can be followed up for longer periods and be approached to participate in related projects as appropriate and subject to funding<br/

Basic and translational models of cooperative oncogenesis

No abstract available

More to life than promotion: Self-initiated and self-resigned career plateaus

Being on a career plateau is widely regarded as an undesirable career experience characterised by a lack of individual proactivity, ability, or opportunity for promotion. In this paper, we present an alternative view arguing that some employees may choose to plateau their careers and deliberately forego opportunities for hierarchical progression. Drawing on in-depth interviews with 75 law enforcement officers in the US and Australia, we explore why they declined a promotion opportunity or elected not to apply for promotion. Drawing on social cognitive career theory, we develop a provisional taxonomy characterised by individual proactivity: self-initiated and self-resigned career plateaus. Specifically, we report how the decision to remain on either of these career plateaus is informed by either the low valence accorded to a promoted position or, paradoxically, the reduced self-efficacy in navigating what is viewed as a flawed promotion system